Duke University
June 13, 2023
Offerd Salary:Negotiation
Working address:N/A
Contract Type:Other
Working Time:Negotigation
Working type:N/A
Ref info:N/A

Duke University and Duke University Health System require all students, faculty and staff to receive the COVID-19 and influenza vaccines. After you have officially accepted a position at Duke, you may request a religious or medical accommodation.


Work Arrangement:

Requisition Number: 232250

Regular or Temporary: Regular


Durham, NC, US, 27710

Personnel Area: UNIVERSITY

Date: May 12, 2023

Duke University:

Duke University was created in 1924 through an indenture of trust by James Buchanan Duke. Today, Duke is regarded as one of America's leading research universities. Located in Durham, North Carolina, Duke is positioned in the heart of the Research Triangle, which is ranked annually as one of the best places in the country to work and live. Duke has more than 15,000 students who study and conduct research in its 10 undergraduate, graduate and professional schools. With about 40,000 employees, Duke is the third largest private employer in North Carolina, and it now has international programs in more than 150 countries.

Occupational Summary

Our research centers around natural genetic variation in fungal species, and we seek to characterize genes and alleles contributing to virulence in fungal pathogens. We employ various laboratory and bioinformatics methods, such as QTL analysis, genomic sequencing, gene manipulation, phenotyping, and gene expression analysis.

Our lab seeks to hire a Research Technician II with a Bachelor's degree in Biology or a related discipline to support research on virulence and drug resistance in fungal pathogens and fungal predation by amoebae. This is an ideal position for a recently graduated student interested in lab experience before graduate school or a career in biotech.

A successful candidate will be highly organized, detail-oriented, self- motivated and possess excellent communication skills. Experience with standard techniques used in fungal research and gene manipulation is desirable but not essential.

Work Performed

Key responsibilities:

  • Use gene manipulation techniques such as CRISPR to investigate gene function (primer design, PCR, gene disruption, confirmation, Sanger sequencing, phenotyping)
  • General lab support (maintaining stocks, media, protocols, etc)
  • Research support as needed for ongoing laboratory experiments.
  • General description of work performed:

    Perform a variety of complex technical duties involved in conducting experiments; collect, organize, and analyze data, document and draw tentative conclusions from experimental results.

    Confer with lab personnel to review work assignments and develop plans for research experiments; maintain laboratory notebook documenting experiments; write-up laboratory protocols.

    Develop and troubleshoot a standard laboratory protocol for genetic manipulations using CRISPR/Cas9; perform and troubleshoot PCR methods to accumulate genetic material and construct needed cassettes; verify results with PCR and sequencing.

    Review laboratory methods manual, scientific journals, abstracts and other literature for information applicable to research experiments; assist in basic laboratory maintenance; participate in data analysis, presentation and manuscript preparation.

    Follow standard lab safety protocols and SOPS for handling of fungal pathogens and hazardous chemicals; assist in treatment and disposal of biohazard materials; practice standard sterile techniques in all laboratory experiments.

    Perform other related duties incidental to the work described herein.

    The above statements describe the general nature and level of work being performed by individuals assigned to this classification. This is not intended to be an exhaustive list of all responsibilities and duties required of personnel so classified.

    Minimum Qualifications


    Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.


    None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

    Job Code: 00000951 RESEARCH TECHNICIAN II Job Level: 08

    Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

    Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

    Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

    Nearest Major Market: Durham Nearest Secondary Market: Raleigh

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