Research Associate (Levels II and III) - Zhou Lab - Buck Institute -
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Protein Biochemistry and IDR Sample Production
POSITION SUMMARY
The Zhou Lab at the Buck Institute for Research on Aging is seeking an
experienced Research Associate (level II and III) to support a
multidisciplinary research program investigating the conformational and
functional biology of intrinsically disordered proteins and regions
(IDPs/IDRs).
This is an experienced research position intended for a scientist capable of
independently executing and optimizing technically demanding experimental
workflows. The Research Associate will work closely with postdoctoral
researchers and other members of the project team and will play a major role
in large-scale protein production, quality control, sample preparation, and
biochemical characterization.
KEY RESPONSIBILITIES
Carry out protein expression, purification, and quality control for a
large collection of IDR-containing proteins in human cells.
Optimize purification and handling protocols for proteins that may be
unstable, aggregation-prone, highly charged, or structurally
heterogeneous.
Perform routine and advanced protein characterization and maintain
standardized QC criteria across protein batches.
Prepare and document samples for transfer to collaborating laboratories
performing single-molecule profiling, HDX-MS, protein-interactome, and
related assays.
Support in vitro protein interaction, condensation, aggregation, and
binder-characterization experiments.
Maintain detailed sample inventories, batch records, QC documentation, and
transfer manifests.
Assist with implementation and scaling of reproducible experimental
workflows.
Work closely with the postdoctoral fellow to troubleshoot experimental
problems and improve throughput and reproducibility.
Contribute to preparation of technical reports and milestone
documentation.
REQUIRED QUALIFICATIONS
Bachelor's or Master's degree in biochemistry, molecular biology,
biotechnology, chemistry, protein science, or a related field, with
substantial relevant research experience.
Extensive hands-on experience in recombinant protein expression and
purification.
Demonstrated ability to independently execute established protocols and
troubleshoot technically challenging experiments.
Strong laboratory organization, record keeping, and sample-management
skills.
Experience managing multiple experimental samples or workflows in
parallel.
Ability to work collaboratively in a fast-moving multidisciplinary
research environment.
PREFERRED QUALIFICATIONS
Direct experience purifying and handling intrinsically disordered proteins
or IDR-containing proteins is highly preferred.
Experience with difficult, aggregation-prone, low-complexity, or phase-
separating proteins.
Experience with affinity chromatography, ion exchange, SEC, protein
concentration/formulation, electrophoresis, and quantitative protein
assays.
Experience preparing samples for mass spectrometry, HDX-MS, structural
biology, or other high-end analytical platforms.
Experience establishing standardized protein-production or QC workflows.
Familiarity with automation or high-throughput protein production would be
advantageous.
This position would be an excellent fit for an experienced research
professional who enjoys hands-on experimental work and building reliable
protein-production pipelines.
COMPENSATION & BENEFITS
Annual salary: RA II $65,000 - $80,000; RA III $70,500 - $90,000 depending
on skills and experience
Comprehensive benefits including medical/dental/vision coverage, paid time
off, paid parental leave, 401(k) employer contribution, and a student
debt repayment option.
ABOUT THE BUCK
The Buck Institute for Research on Aging is the first independent biomedical
research institute in the United States devoted solely to research on aging.
Located in Novato, California, the Buck brings together scientists across
multiple disciplines to study the mechanisms of aging and age-related disease
to help people live better, longer.
The Buck Institute is an equal opportunity employer. We strongly encourage
applications from candidates whose experiences and perspectives will
contribute to the diversity and excellence of our community.
TO APPLY
Please submit online:
A curriculum vitae or resume
A cover letter addressing your past research experience, plans, and
expectations for working in the lab
