Research Assistant

Posting Details

I. DEPARTMENT INFORMATION

Job Description Summary:

GW's Department of Microbiology, Immunology, and Tropical Medicine (MITM) conducts innovative, fundamental, basic and translational research for HIV/AIDS and neglected tropical diseases. MITM has a strong tradition of training and policy advocacy for these infectious and parasitic diseases, which initiate and perpetuate poverty and suffering worldwide. Additional information on MITM can be found at the following link: https:// smhs.gwu.edu/microbiology/

MITM is in search of a full-time Research Assistant for Dr. Hovel- Miner's laboratory. The Hovel-Miner Laboratory is using innovative genetic tools to investigate gene functions in the trypanosomatid parasites. Specifically, in the African trypanosome (T. brucei spp.) the lab is using forward genetic methods to identify genes that promote drug resistance and elucidating genetic regulatory pathways that lead to parasitic survival during drug treatment. Currently, we are investigating novel genes that effect redox and ROS biology in the mitochondrion. The laboratory consists of interdependent work platforms that include basic molecular biology, parasite genetics, parasite phenotypic analysis (using an array of assays), and next- generation sequencing based genetic screens that are analyzed bioinformatically.

This research support position contributes to the research and development of the Hovel-Miner Lab by assisting with ongoing and independently developed experiments. The core responsibilities of this position include assisting with the execution of experiments planned by Dr. Hovel-Miner, or other senior staff, data collection, supervised data analysis, and detailed notes and record keeping. The position may assist with preparation of figures for presentation, manuscript publication, or grant submissions. The position also includes lab management, supply ordering, and day to day upkeep of laboratory equipment.

Additional Lab Duties/ Responsibilities Include:

Sterile technique

Assist in growth and maintenance of Trypanosoma brucei in cell culture.

Bacterial growth

Assist in nucleic acid isolations: genomic DNA, plasmid DNA, and RNA.

Assist with flow-cytometry and other cell biology approaches

Assist in T. brucei cell line generation / mutational analyses

Assist in mid-range scale forward genetic screens (liters of cells grown)

Assist with planning and scheduling experiments consistent with the timeline and objectives of ongoing research projects.

Assist with routine general molecular biology approaches such as PCR, gel electrophoresis, and restriction digests.

Assist in preparation of DNA libraries for next-generation sequence analysis.

Assist in data management and analysis

Perform preparation of specialized T. brucei media.

Assist in general laboratory upkeep.

Assist in ordering supplies and maintaining lab stocks.

Assist in equipment maintenance.

Keep meticulous scientific records in a digital notebook.

Participate in regular lab meetings.

Performs other related duties as assigned. The omission of specific duties does not preclude the supervisor from assigning duties that are logically related to the position.

Minimum Qualifications:

Qualified candidates will hold a BA/BS in a related discipline. Degree must be conferred by the start date of the position.

Additional Required Licenses/Certifications/Posting Specific Minimum Qualifications: Preferred Qualifications:

Bachelor's degree in biology, chemistry, microbiology, or related field

Laboratory research experience

Good aseptic technique

Experience in bacterial growth and media preparation

Experience in nucleic acid extractions, PCR, and gel electrophoresis

Molecular cloning experience a plus

Preparation of next-generation sequencing samples is a plus

Experience with bioinformatic analyses

Biopython coding experience is a plus

Strong written and verbal communication skills highly desired

Ability to work well with others on a scientific team highly desired

Typical Hiring Range Commensurate with Experience.

II. POSITION INFORMATION

Campus Location: Foggy Bottom, Washington, D.C.

College/School/Department: School of Medicine and Health Sciences (SMHS) Family Research and Labs Sub-Family Laboratory Research Stream Service and Support Level Level 2 Full-Time/Part-Time: Full time -> FTE 1.00 Hours Per Week: 40 Work Schedule: Monday through Friday, 9:00am to 5:00pm Will this job require the employee to work on site? Yes Employee Onsite Status On-campus (in person) Telework: Required Background Check Criminal History Screening, Education/Degree/Certifications Verification, Social Security Number Trace, and Sex Offender Registry Search Special Instructions to Applicants:

- Employer will not sponsor for employment Visa status - The position is a limited term assignment ending on June 30, 2025

Internal Applicants Only? No Posting Number: R001983 Job Open Date: 07/26/2023 Job Close Date: If temporary, grant funded or limited term appointment, position funded until: 06/30/2025 Background Screening Successful Completion of a Background Screening will be required as a condition of hire. EEO Statement:

The university is an Equal Employment Opportunity/Affirmative Action employer that does not unlawfully discriminate in any of its programs or activities on the basis of race, color, religion, sex, national origin, age, disability, veteran status, sexual orientation, gender identity or expression, or on any other basis prohibited by applicable law.

Posting Specific Questions

Required fields are indicated with an asterisk ().

Are you enthusiastic about conducting molecular biology and genetics research?

(Open Ended Question)

Do you prefer to work collaboratively or independently?

(Open Ended Question)

Do you enjoy working in a laboratory research setting?

(Open Ended Question)

Do you enjoy mentoring students?

(Open Ended Question)

Applicant Documents

Required Documents

Resume

Cover Letter

Optional Documents