The Role Of Brm Atpase Interplay With Minu1 And Minu2 Atpases In Organ-Specific Alternative Rna...

Universities and Institutes of Poland

Poland

August 15, 2022

Description

  • Organisation/Company: Institute of Biochemisty and Biophysics Polish Academy of Sciences
  • Research Field: Biological sciences
  • Researcher Profile: First Stage Researcher (R1)
  • Application Deadline: 15/08/2022 23:00 - Europe/Athens
  • Location: Poland › Warsaw
  • Type Of Contract: Other
  • Job Status: Full-time
  • Hours Per Week: 40
  • Offer Starting Date: 01/10/2022
  • Reference Number: DSMBBC/2022/15
  • The SWI/SNF class of ATP-dependent chromatin remodeling complexes (CRCs), evolutionary conserved among eukaryotes, has been the subject of research throughout the world for more than three decades. The SWI/SNF is involved in regulating various processes such as gene expression control or alternative splicing, control of developmental program, etc. However, from a developmental point of view, the crucial function of these complexes in the organ-specific alternative transcript formation is still unknown. The Arabidopsis SWI/SNF complexes contain one of the four ATPases: BRM, SYD, MINU1, or MINU2. Double mutant syd/brm and minu1/minu2 are lethal. Most research in alternative transcript formation regulated by SWI/SNF complex in Arabidopsis concerns BRM containing SWI/SNF complex class and doesn't describe organ-specific function. So far, no literature data is providing any information about the role of MINU1 and MINU2 ATPases in the organ-specific alternative transcription regulation in Arabidopsis as well as there is no data about the possible characteristic traits of minu1 or minu2 mutant in brm background and potential replacement of MINU1 or MINU2 functions by BRM ATPase. The presented project aims to verify new, hitherto unknown functions of SWI/SNF small ATPases, MINU1 and MINU2, in the organ-specific alternative RNA processing regulation and the possible interplay with BRM ATPase functions in model plant Arabidopsis which represents an innovative and cutting-edge approach of research. To reach the primary goal, the four following research tasks with an innovative approach are proposed:

    1) Influence of BRM ATPase inactivation on MINU1 and MINU2 organ-specific function in canonical and alternative RNA processing regulation;

    2) The direct and indirect influence of MINU1 and MINU2 ATPases on the organ- specific alternative RNA processing and gene expression regulation;

    3) Assessment of the MINU1 and MINU2 impact on chromatin structure and its influence on the regulation of alternative transcription and gene expression regulation;

    4) Analysis of organ-specific translationally active alternative mRNA.

    To successfully execute the presented project, the most modern molecular biology methods such as next-generation sequencing analysis (RNA-seq, Chip- seq, MNase-seq, and ChIP- BS-seq) are planned to perform. Moreover, to get a more reliable result, the following confirmatory methods: ChIP-qPCR, MNase- qPCR, and "Methylation Specific qPCR” will be used. Finally, the Arabidopsis lines expressing proteins MINU1, MINU2, and BRM tagged with GFP or mCherry tag etc. The T-DNA insertional Arabidopsis mutants – minu1, minu2, brm as well as the double mutant: minu1/brm, minu2/brm. Such research approach will allow to determine the significance of biological function of MINU1 and MINU2 as well as interplay with BRM and SYD functions in organ-specific alternative transcription regulation. Moreover, the results of global analysis will be used for comparative analysis of DNA methylation and histone modifications and corelate with the alternative transcripts formation to uncover the mechanism of MINU1 and MINU2 dependent organ-specific alternative transcription regulation in Arabidopsis.

    Selection process

    The recruitment process consists of two stages:

    a) selection of candidates by the Committee based on their previous achievements and academic performance presented in the documents submitted; for each position no more than 4 applicants who have achieved the highest scores, but no less than 60% of the maximum points, shall be qualified for the next stage;

    b) an interview conducted by the Committee including in particular:

    • a presentation delivered by the candidate containing the outcomes of his/her research (a Master's thesis or other research work carried out by the candidate); the presentation must not last longer than 10 minutes;

    • questions asked by the members of the Committee related to the presented project, the methods used and interpretation of the results obtained;

    • questions asked by the members of the Committee related to the proposed PhD programme described in the recruitment announcement;

    • questions related to the candidate's motivation for scientific work.

    Additional comments

    How to apply:

    • Use the Application form for admission to the Doctoral School (link to the form)

    • Merge all required documents as a single pdf file

    • Send this file as an attachment to PhDschool-recruitment@ibb.waw.pl or post documents to

    Doctoral School (room 41)

    Institute of Biochemistry and Biophysics

    Polish Academy of Sciences

    Pawinskiego 5a

    02-106 Warszawa

    Poland

    Include "Procedure no. DSMBBC/2022/13" and your first and last name as the subject of the e-mail

    The application must contain all required documents.

    For additional information please contact:

  • formal issues: PhDschool-recruitment@ibb.waw.pl (Research Management Unit IBB PAS);
  • PhD programme-related issues: the supervisor at IBB PAS (we encourage you to contact the supervisor to learn more about the projectbefore submitting your application)
  • Required Research Experiences
  • RESEARCH FIELD
  • Biological sciences

  • YEARS OF RESEARCH EXPERIENCE
  • 1 - 4

    Offer Requirements
  • REQUIRED EDUCATION LEVEL
  • Biological sciences: Master Degree or equivalent

  • REQUIRED LANGUAGES
  • ENGLISH: Good

    Skills/Qualifications

    Full offer DSMBBC/2022/15

    Requirements for the candidate (wymagania):

    a) hold a degree of Master of Science Magister, Master of Engineering Magister Inżynier, medical doctor or equivalent in the field of: exact sciences, natural sciences, medical sciences or related disciplines, granted by a Polish or foreign university; a person who does not possess the qualifications described above may take part in the competition, but must obtain the qualifications in question and provide the relevant documents before the start of the programme at the Doctoral School (i.e., October 1, 2022);

    b) Good or very good knowledge of English

    c) Knowledge in chromatin remodeling, transcription control, alternative transcription, etc.

    d) Knowledge of basic molecular biology techniques is welcome (cloning, RT qPCR etc.)

    e) Good practice in laboratory work

    f) Motivation for work with the agreement of scientific ethic

    g) Good communication skills with other Lab members

    h) The research publications are welcome

    Specific Requirements

    Required documents:

    a) an application for admission to the Doctoral School, together with consent to the processing of personal data for the recruitment procedure and a statement on familiarising oneself with recruitment rules and conditions; (link to the form)

    b) a copy of the diploma from a university or the candidate's statement on the estimated graduation date;

    c) a scientific curriculum vitae including information on the candidate's participation in scientific projects and other related

    activities, such as publications, involvement in science club, participation in academic conferences with an oral or poster

    presentation, national and international internships, prizes and awards, participation in grants, popularization of science, voluntary work;

    d) a list of completed university courses with marks;

    e) a motivation letter;

    f) contact details to at least one academic adviser or academic staff member holding at least a doctor's degree, who has agreed to give an opinion on the applicant. The opinion should not be attached to the application;

    g) in the case of having a disability certificate or a certificate on the degree of disability, or a certificate referred to in Article 5 and Article 62 of the Act of 27 August 1997 on Vocational and Social Rehabilitation and Employment of Persons with Disabilities ‒ a statement of holding such certificate;

    h) candidates holding a diploma of completing studies abroad shall additionally attach a certified translation of the diploma, including the supplement, into Polish or English, unless the diploma or an official copy thereof, including the supplement, has been issued in English.

    Language of documents:

    • either English or Polish up to the candidate's choice

    Contact Information
  • Organisation/Company: Institute of Biochemisty and Biophysics Polish Academy of Sciences
  • Department: Laboratory of Gene Expression Regulation
  • Organisation Type: Research Laboratory
  • Website: https:// www. ibb.edu.pl https: // ibb.edu.pl/en/laboratory/tomasz-j-sarnowski/
  • E-Mail: tsarn@ibb.waw.pl
  • Country: Poland
  • City: Warsaw
  • Postal Code: 02-106
  • Street: ul. Pawińskiego 5a
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