Researcher in mass spectrometry based PTM proteomics

Lund University

Sweden

September 23, 2021

Description

Lund University, Faculty of Engineering, Institutionen för immunteknologi

Lund University was founded in 1666 and is repeatedly ranked among the world's top 100 universities. The University has around 44 000 students and more than 8 000 staff based in Lund, Helsingborg and Malmö. We are united in our efforts to understand, explain and improve our world and the human condition.

LTH forms the Faculty of Engineering at Lund University, with approximately 9 000 students. The research carried out at LTH is of a high international standard and we are continuously developing our teaching methods and adapting our courses to current needs.

Subject description The employment mainly involves research related to proteomic analyses of post translational modifications (PTM). The project is a collaboration between Magnus Jakobssons research group at the Department of Immunotechnology, Lund University, and Börje Sellergrens research group at the Department of Biomedical sciences, Malmö university.

The project strives to develop and characterize new affinity agents for enrichment of proteins and peptides carrying PTMs for analysis by mass spectromery. Börje Sellergren has expertize regarding the development of molecularly imprinted polymers (MIPs) for enrichment of PTM containing peptides. Magnus Jakobssons group has complementary expertize regarding analysis of modified peptides by quantitative mass spectrometry. The project is anticipated to be carried out to a degree of 50 % at Lund university and 50 % at Malmö university.

Research concerning mass spectrometry at the deaprtment of Immunotehcnology is linked to the infrastructure Proteoforms@LU. Proteoforms@LU contributes advanced liquid chromatography mass spectrometry (LC-MS) equipment, as well as experience concerning preparation of samples for analysis. Used protocols often include different affinity based methods, particularly enrichment of PTM containing peptides. Proteoforms &LU works widely with PTMs but focuses on methylation and phosphorylation. A main focus of the infrastructure is further development of automated sample preparation for reprodcible and large scale proteomics studies.

Work duties

The work duties encompass e.g.:

  • Development of new MIPs for affinity enrichment of PTMs for analysis by mass spectrometry.
  • Sample preparation using various protocols and subsequent LC-MS analysis.
  • Analysis of data correpsonding to the above.
  • Possibility to supervise degree students and PhD students.
  • Possibility to apply for external resarch funding.
  • Administration relatd to the tasks above.
  • Qualification requirements

  • A PhD degree in a relevant subject such as biochemistry, cell biology, or equivalent.
  • Proficiency in English.
  • Experience in development of PTM MIPs.
  • Experience in enrichment and analysis pf PTMs.
  • Experience with automated sample preparation for mass spectrometry.
  • Assessment criteria and other qualifications

  • Programming and statistical analysis with Python, R, or similar.
  • Regard will be taken to cooperative ability, drive and independence as well as how the applicant through his, or her, experience is judged to complement and strengthen ongoing research at the department and contribute to its future development.

    Terms of employment The position as researcher involves employment to the degree of 50 % at Lund university and 50 % at Malmo university.

    Instructions for applications The application shall be written in English and contain:

  • CV and list of publications.
  • A general description of earlier research and planned future research (max 3 pages).
  • Contact details for at least two references.
  • Copy of PhD degree diploma as well as potential other documentation the candidate wishes to have considered.
  • Lund University welcomes applicants with diverse backgrounds and experiences. We regard gender equality and diversity as a strength and an asset. We kindly decline all sales and marketing contacts.

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    Type of employment Temporary position longer than 6 months

    Contract type Full time Number of positions 1 Working hours 100% City Lund County Skåne län Country Sweden Reference number PA2021/2880 Contact

  • Magnus Jakobsson, 0735 25 47 00
  • Börje Sellergren, 040-6657810
  • Union representative

  • OFR/ST:Fackförbundet ST:s kansli, 046-222 93 62
  • SACO:Saco-s-rådet vid Lunds universitet, 046-222 93 64
  • SEKO: Seko Civil, 046-222 93 66
  • Published 09.Sep.2021 Last application date 23.Sep.2021 11:59 PM CEST

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