Research Technician – Regulation of Chloroplast Protein Import
Department of Plant Sciences, South Parks Road, Oxford OX1 3RB
Grade 5: £26,341 - £31,406 per annum
The Department of Plant Sciences is seeking to recruit one Research Technician
for 6 months. This post is financially supported by the John Fell Fund held by
Dr Francesco Licausi. The work is to be conducted in his lab in the Department
of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB.
The research topic is ‘The crystal structure of RAP2.12 DNA binding domain'.
Adaptation to hypoxia in higher plants is mediated by the group VII of
Ethylene Response Factors (ERFs), which modulate the transcriptional
response to hypoxia. Whereas the vast majority of ERF proteins recognise a DNA
element defined as the GCC-box, group VIIs preferentially regulate genes whose
promoter contains a more complex sequence, defined as the Hypoxia Responsive
Promoter Element (HRPE). Preliminary observations suggest that novel
domains, in addition to the traditional AP2 responsible for binding the GCC-
box, are required in vivo for DNA recognition by RAP2.12, the main ERF-VII
transcription factor of Arabidopsis thaliana. However, conclusive evidence
for this can only come from the structural characterisation of the
nucleoprotein complex. Therefore, we seek for a research technician to help
with RAP2.12 protein expression, purification testing and crystallization of
the RAP2.12 fragment that is sufficient for HRPE binding. Completion of this
project will lead to publication of the results in a peer reviewed research
journal. Moreover, we envisage many possibilities for future exploitation of
the results obtained in this project: modulation of ERF activity over specific
targets in crops; studying the development of ERF-VII and the anaerobic
response throughout the evolution of land plants; and analysis of how amino
acid substitutions affect nucleotide recognition in other ERF proteins.
Duties: The research technician will carry out experiments and analyse data.
They will also be involved in presenting and discussing their results within
the group. Presentation and critical discussion of results and related
literature will also be fostered during group meetings. The research
technician is also expected to contribute to lab and group management.
The postholder should have a master degree or equivalent (or be very close to
obtaining one) in a relevant area. A publication record, including
manuscripts deposited in preprint servers, although welcome, is not required.
Successful candidates are expected to demonstrate intellectual curiosity and
rigour, ability to work independently and manage multiple tasks as well as
previous experience with protein expression and purification.
The closing date for applications is 12.00 noon on Tuesday 21 December 2021.
Informal enquiries should be directed to at email@example.com.
Excellent benefits include 38 days' leave. A full-time position, 36.5 hours
Contact Person : Recruitment Administrator Vacancy ID : 154552
Contact Phone : 01865 275000 Closing Date & Time : 21-Dec-2021 12:00
Contact Email : firstname.lastname@example.org