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Research Technician II - Breeding Core, DLAR
Requisition Number:: 171949
Date: Jan 5, 2022
Durham, NC, US, 27710
Personnel Area: MEDICAL CENTER
School of Medicine:
Established in 1930, Duke University School of Medicine is the youngest of the
nation's top medical schools. Ranked tenth among its peers, the School takes
pride in being an inclusive community of outstanding learners, investigators,
clinicians, and staff where traditional barriers are low, interdisciplinary
collaboration is embraced, and great ideas accelerate translation of
fundamental scientific discoveries to improve humanhealth locally and around
Comprised of 2,400 faculty physicians and researchers, the Duke University
School of Medicine along with the Duke University School of Nursing and Duke
University Health System create Duke Health. Duke Health is a world-class
health care network. Founded in 1998 to provide efficient, responsive care,
the health system offers a full network of health services and encompasses
Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke
Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health
and Wellness, and multiple affiliations.
Perform a variety of technical duties involved in basic through advanced
breeding colony management.
Special Conditions of Employment
Must be able to work a flexible working schedule based on workload and
Must be able to work assigned standard schedules, including (but not
limited to) Mon-Fri, Wed-Sun and Tue-Sat shifts;
Must be able to work on rotating on call duties after hours and on
Occupational Health and Safety
As a safeguard for the employee and the animals, Veterinary and Research
Technicians may be subject to vaccines, tests, physical examinations prior to
working with specific animals or animal populations as required by research
needs, including, but not limited to:
TB testing and clearance;
Hepatitis B (proof of immunization and/or vaccination);
Rabies (proof of immunization and/or vaccination);
Tetanus (current vaccination less than 10 years);
Measles (proof of immunization and/or vaccination);
Periodic physical examinations including respiratory fit tests);
Other vaccines or testing as required by EOHW standards.
The animal facility has a variety of environmental conditions. Specifically,
employees may be exposed to potentially hazardous noise, dust, chemicals,
extreme heat, and animal waste products. The duration of exposure of any of
the above is dependent on assigned duties. Some aspects of this position may
expose the incumbent to potentially zoonotic and other biohazardous agents.
Appropriate protective clothing and equipment may be required and will be
provided when required, accompanied by appropriate training.
Manages breeding colonies with the general goal to provide healthy,
genetically well-defined mice that are suitable for research in an
efficient and cost-effective manner and serves as liaison and expert in
the field for rodent colony management:
Researches mouse strains genotypes and phenotypes to develop the best
strategies for setting up and monitoring breeding and optimize colony
Develops timelines in regards to colony production for core investigators
and delivers animal subjects to core investigators for experimental use
based on their needs.
Tracks pedigrees of strains and develops strategy for strict genetic
quality control including managing and preventing genetic contamination
and genetic drift.
Manages weaning of colonies for research.
Communicates with core investigators regarding their lines and study needs
and informs on progress of breeding colony management.
Communicates with Breeding Core manager regarding censuses, line specific
concerns, and maintenance of lines.
Researches white papers in regards to current techniques in breeding,
cryopreservation, PCR, genotyping, phenotyping, superovulation, and
collection of gametes from donor mice.
Sets up instruments for PCR (thermal cycler, electrophoresis, gel
camera) and associated media such as microscopy, sperm analysis (visual
and computerized), operation and maintenance of liquid nitrogen freezers,
anesthesia machines, instrument cleaning and sterilization tattoo machine.
Performs tail biopsies, ear punch biopsies, ear punching/tagging, rodent
tattooing, blood collection for genotyping, as well as organ and fecal
collections for investigators.
Maintains inventory of animals, ensures mouse order fulfillment and does
forecasting for PIs.
Performs on-call requirements as assigned which include weekend and
Assists in and performs a variety of complex technical activities involved
in research support and in the care of research and production colonies.
Determines and sets up instruments, materials, and apparatuses; operates
laboratory equipment required for specific tests.
Prepares culture media, chemicals, reagents, and solutions related to
Maintains sufficient inventory of materials, supplies, and equipment for
performance of duties; cleans and maintains laboratory equipment.
Conducts and helps maintain training program for DLAR and scientific
Reviews work of lower level laboratory personnel within designated areas
and assists in training new personnel.
Participates in outreach with research laboratories through brown bag
seminars, Mouse IACUC Workshops, and others as needed.
Budget and Recharges
Performs responsible tracking and submission of service requests invoices
and recharges to the breeding core manager and others parties.
Maintains appropriate documentations for all duties in accordance with all
applicable departmental, institutional, local, state and federal laws,
regulations, policies, guidelines and SOPs, and based on contemporary
Professional Conduct and Behavior
Demonstrates professional conduct and behavior at all times when
performing job duties and rendering services to the scientific community,
especially as they relate to customer service, attendance and punctuality,
respectful demeanor and interactions, professional language and
communications, appropriate effort and initiative, accountability and
integrity, boundary maintenance and confidentiality, emotional self-
regulation and responsiveness to feedback.
Maintains professional standards and adheres to all federal, state and
university regulations and policies regarding the use of animals in
research while when performing job duties.
Fosters teamwork and collegiality between the various groups of DLAR and
Performs other related duties incidental to the work described herein as
assigned by the supervisor based on operational needs.
DLAR Basic Skills:
Clinical observations & evaluations of animal health status
Assist in surgery
Anesthetize, catheterize, and intubate a variety of animals
Proficient at basic rodent technical skills (IP, SQ, IV injections, blood collection, etc.)
Administer daily treatments and medications
Basic animal restraint techniques
Basic diagnostic lab techniques
Basic radiograph techniques
Participate in enrichment plan
Work generally requires a bachelor's degree in botany, biology, zoology,
psychology or other directly related scientific field.
None required above education/training requirement. OR AN EQUIVALENT
COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE
Duke is an Affirmative Action/Equal Opportunity Employer committed to
providing employment opportunity without regard to an individual's age, color,
disability, gender, gender expression, gender identity, genetic information,
national origin, race, religion, sex, sexual orientation, or veteran status.
Duke aspires to create a community built on collaboration, innovation,
creativity, and belonging. Our collective success depends on the robust
exchange of ideas—an exchange that is best when the rich diversity of our
perspectives, backgrounds, and experiences flourishes. To achieve this
exchange, it is essential that all members of the community feel secure and
welcome, that the contributions of all individuals are respected, and that all
voices are heard. All members of our community have a responsibility to uphold
Essential Physical Job Functions: Certain jobs at Duke University and Duke
University Health System may include essentialjob functions that require
specific physical and/or mental abilities. Additional information and
provision for requests for reasonable accommodation will be provided by each
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