Research Technician II - Breeding Core, DLAR

Duke University

United States

February 6, 2022

Description

Duke University and Duke University Health System require all students, faculty and staff to receive the COVID-19 and influenza vaccines. After you have officially accepted a position at Duke, you may request a religious or medical accommodation.

Research Technician II - Breeding Core, DLAR

Requisition Number:: 171949

Date: Jan 5, 2022

Location:

Durham, NC, US, 27710

Personnel Area: MEDICAL CENTER

School of Medicine:

Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.

Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Job Summary

Perform a variety of technical duties involved in basic through advanced breeding colony management.

Special Conditions of Employment

Working Schedule

  • Must be able to work a flexible working schedule based on workload and deadlines;
  • Must be able to work assigned standard schedules, including (but not limited to) Mon-Fri, Wed-Sun and Tue-Sat shifts;
  • Must be able to work on rotating on call duties after hours and on weekends;
  • Occupational Health and Safety

    As a safeguard for the employee and the animals, Veterinary and Research Technicians may be subject to vaccines, tests, physical examinations prior to working with specific animals or animal populations as required by research needs, including, but not limited to:

  • TB testing and clearance;
  • Hepatitis B (proof of immunization and/or vaccination);
  • Rabies (proof of immunization and/or vaccination);
  • Tetanus (current vaccination less than 10 years);
  • Measles (proof of immunization and/or vaccination);
  • Periodic physical examinations including respiratory fit tests);
  • Other vaccines or testing as required by EOHW standards.
  • Hazard Exposure

    The animal facility has a variety of environmental conditions. Specifically, employees may be exposed to potentially hazardous noise, dust, chemicals, extreme heat, and animal waste products. The duration of exposure of any of the above is dependent on assigned duties. Some aspects of this position may expose the incumbent to potentially zoonotic and other biohazardous agents. Appropriate protective clothing and equipment may be required and will be provided when required, accompanied by appropriate training.

    Work Performed

    Breeding Services

  • Manages breeding colonies with the general goal to provide healthy, genetically well-defined mice that are suitable for research in an efficient and cost-effective manner and serves as liaison and expert in the field for rodent colony management:
  • Researches mouse strains genotypes and phenotypes to develop the best strategies for setting up and monitoring breeding and optimize colony production.
  • Develops timelines in regards to colony production for core investigators and delivers animal subjects to core investigators for experimental use based on their needs.
  • Tracks pedigrees of strains and develops strategy for strict genetic quality control including managing and preventing genetic contamination and genetic drift.
  • Manages weaning of colonies for research.
  • Communicates with core investigators regarding their lines and study needs and informs on progress of breeding colony management.
  • Communicates with Breeding Core manager regarding censuses, line specific concerns, and maintenance of lines.
  • Researches white papers in regards to current techniques in breeding, cryopreservation, PCR, genotyping, phenotyping, superovulation, and collection of gametes from donor mice.
  • Sets up instruments for PCR (thermal cycler, electrophoresis, gel camera) and associated media such as microscopy, sperm analysis (visual and computerized), operation and maintenance of liquid nitrogen freezers, anesthesia machines, instrument cleaning and sterilization tattoo machine.
  • Performs tail biopsies, ear punch biopsies, ear punching/tagging, rodent tattooing, blood collection for genotyping, as well as organ and fecal collections for investigators.
  • Maintains inventory of animals, ensures mouse order fulfillment and does forecasting for PIs.
  • Performs on-call requirements as assigned which include weekend and holiday rotations.
  • Technical Services

  • Assists in and performs a variety of complex technical activities involved in research support and in the care of research and production colonies.
  • Determines and sets up instruments, materials, and apparatuses; operates laboratory equipment required for specific tests.
  • Prepares culture media, chemicals, reagents, and solutions related to laboratory procedures.
  • Maintains sufficient inventory of materials, supplies, and equipment for performance of duties; cleans and maintains laboratory equipment.
  • Training

  • Conducts and helps maintain training program for DLAR and scientific staff.
  • Reviews work of lower level laboratory personnel within designated areas and assists in training new personnel.
  • Participates in outreach with research laboratories through brown bag seminars, Mouse IACUC Workshops, and others as needed.
  • Budget and Recharges

  • Performs responsible tracking and submission of service requests invoices and recharges to the breeding core manager and others parties.
  • Record Keeping

  • Maintains appropriate documentations for all duties in accordance with all applicable departmental, institutional, local, state and federal laws, regulations, policies, guidelines and SOPs, and based on contemporary professional standards.
  • Professional Conduct and Behavior

  • Demonstrates professional conduct and behavior at all times when performing job duties and rendering services to the scientific community, especially as they relate to customer service, attendance and punctuality, respectful demeanor and interactions, professional language and communications, appropriate effort and initiative, accountability and integrity, boundary maintenance and confidentiality, emotional self- regulation and responsiveness to feedback.
  • Maintains professional standards and adheres to all federal, state and university regulations and policies regarding the use of animals in research while when performing job duties.
  • Fosters teamwork and collegiality between the various groups of DLAR and scientific community.
  • Others

  • Performs other related duties incidental to the work described herein as assigned by the supervisor based on operational needs.
  • Skills

    DLAR Basic Skills:

  • Clinical observations & evaluations of animal health status
  • Assist in surgery
  • Anesthetize, catheterize, and intubate a variety of animals
  • Proficient at basic rodent technical skills (IP, SQ, IV injections, blood collection, etc.)
  • Administer daily treatments and medications
  • Basic animal restraint techniques
  • Basic diagnostic lab techniques
  • Basic radiograph techniques
  • Participate in enrichment plan
  • Minimum Qualifications Education

    Work generally requires a bachelor's degree in botany, biology, zoology, psychology or other directly related scientific field.

    Experience

    None required above education/training requirement. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

    Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

    Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

    Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

    Nearest Major Market: Durham Nearest Secondary Market: Raleigh

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