Research Engineer for Clinical Proteomics Mass Spectrometry

Karolinska Institute Stockholm


November 30, 2021


Do you want to contribute to top quality medical research?

At the Department of Oncology-Pathology basic, translational and clinical research and educational activities related to cancer is carried out. Approximately 300 people from over 40 nations are currently working at the department. About 30 research groups with various cancer research profiles are involved and we have around 110 PhD students. The Department of Oncology- Pathology is responsible for undergraduate courses in Pathology, Oncology and Forensic Medicine for medical students, as well as for Tumor biology courses for biomedicine students and Pathology courses for opticians.

The position is within the regional and the national core facilities led by Professor Janne Lehtiö, located at the Science for Life Laboratory in Solna. The regional core facility is tasked with aiding users of Swedish universities and industry with mass spectrometry (MS) based proteomics projects. The national facility (SciLifeLab/BioMS) offers infrastructure for biological mass spectrometry (www. Combined, the regional and national facilities offer a unique research infrastructure and expertise with focus on MS-based proteomics and proteogenomics. The core facility works in close collaboration with Prof. Janne Lehtiö's research group, where MS-based proteomics methods are constantly developed and applied to studies aiming at improving individualized cancer therapy. This collaboration is a prerequisite for the core facility's ability to offer services at the forefront of MS based proteomics.

We are now looking for a PhD-level research engineer with experience of core facility work or equivalent to join the team working in the Mass Spectrometry core facility.

Learn more about the Lehtiö lab and Clinical Proteomics Mass Spectrometry Core Facility.

Your mission

The responsibilities accompanying this position include sample preparation, bio analytical work, analysis and reporting of protein/peptide expression, method development, use/optimization of the MS instruments, data analysis and data interpretation. Experiences in method development, data analysis and reporting are important.

The role also includes reporting analysis results to facility users, taking part of undergraduate/postgraduate teaching/training and representing the facilities at national and international meetings and conferences.

The research engineer will be part of a research infrastructure, working closely with project scientists, postdoctoral fellows, PhD-students, undergraduate students, and external users/customers within academia and industry. Important aspects of the job include:

  • Preparation of clinical and in-vitro samples for proteomics
  • Experimental analysis of samples by mass spectrometry
  • Compiling and analyzing proteomics data
  • Continuous contact with customers by email and phone
  • The work involves a combination of flexible and routine tasks and places a great emphasis on independence, initiative, precision, and planning. The position involves a variety of tasks in a stimulating research environment. Nice and helpful colleagues contribute to a good working environment within the group.

    A large part of the job may be contact with customers/researchers from a wide range of fields, which is an exciting, interesting and instructive part of the work. The facility/research group is located at the Science for Life Laboratory with spacious and modern facilities.

    We are seeking a highly motivated research engineer to be part of our mass spectrometry core facility.

    Your profile

    The successful candidate needs to meet our requirements.


  • PhD degree in analytical chemistry, biochemistry, biotechnology or in a related field
  • Years of experience in core facility work
  • Extensive hands-on experience of laboratory work and ability to follow SOPs
  • Experience with techniques applied to sample preparation for proteomics, including protein extraction, clean-up ( e.g. by solid phase extraction), protein concentration measurement, as well as protein depletion and/or equalization methodologies and protein and peptide separation/fractionation using gel-based electrophoresis (1D, 2D and HiRIEF).
  • Hands-on experience with Single-Pot Solid-Phase-enhanced Sample Preparation (SP3) and in gel/in solution protein reduction/alkylation and protein digestion techniques
  • Practical experience in liquid chromatography mass spectrometry (LC-MS)
  • Experience in Mass spectrometry Maintenance and Troubleshooting
  • Experience in quantitative MS-based proteomics (TMT10 plex, TMT16 plex)
  • Good knowledge in methods used for analysis of omics data
  • Excellent communication and organizational skills
  • Good knowledge (both speaking and writing) of English
  • Merit:

  • Competence/experience in MS-based clinical proteomics research
  • Competence/experience in cell culture.
  • Competence in sample preparation and mass spectrometry analysis of native peptides and immunopeptidome
  • Collaborative experience with hospitals and clinical sample handling.
  • Data management and data services
  • Strong interests of working in centralized shared research infrastructures
  • We place great emphasis on personal suitability. To succeed in the role, it is important that the applicant has a personal drive and flexible mind to handle the rapidly developing field of proteomics and the need of custom-based solutions in many projects. The applicant should be responsible, appreciate daily detailed work as well as have the ability to see the big picture. The applicant should be an effective communicator, have good organizational skills, and be able to prioritize at times when the workload is high. The applicant should be able to collaborate with people of different backgrounds and at different levels, both within the group and with users from various disciplines.

    What do we offer?

    Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote better health for all. At Karolinska Institutet, we conduct innovative medical research and provide the largest range of biomedical education in Sweden. Karolinska Institutet is a state university, which entitles employees to several benefits such as extended holiday and a generous occupational pension. Employees also have free access to our modern gym and receive reimbursements for medical care.


    Welcome to apply at the latest 30th of November.

    The application is to be submitted through the Varbi recruitment system. Permanent positions is initiated by a six months trial period.

    Want to make a difference? Join us and contribute to better health for


    Type of employment Permanent position
    Contract type Full time
    First day of employment 2022-01-01
    Salary Monthly salary
    Number of positions 1
    Working hours 100 %
    City Solna
    County Stockholms län
    Country Sweden
    Reference number STÖD 2-4906/2021
    • Helena Bäckvall, 08-524 812 09
    • Lukas Orre, 08-517 763 70
    Union representative
    • Andreas Lundqvist, SACO,
    • Henry Wölling, SEKO,
    • Helen Eriksson, OFR,
    Published 10.Nov.2021
    Last application date 30.Nov.2021 11:59 PM CET

    Similar Jobs

    Texas Tech University

    United States Nov 2, 2021

    Add to favorites

    Research Associate Professor

    Contributes to the university's mission through teaching, research and at Texas Tech University (TTU), seeks a non-tenure-track faculty member with We are seeking outstanding candidates with expertise in mass spectrometry and protein and/or small molecule...

    Copenhagen University

    Denmark Nov 3, 2021

    Add to favorites

    Postdoctoral fellow in multi-omics investigation of cardiac disease in humans

    The Cardiac Proteomics Group at Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen wishes to appoint a talented postdoctoral fellow for a 2-year position for a joint proteomics, based approaches...

    University of Cambridge

    United Kingdom Nov 18, 2021

    Add to favorites

    Proteomics Bioinformatician (Fixed Term)

    The Cambridge Institute for Medical Research (CIMR) is a cross-departmental Medicine, CIMR is located in a dedicated research building on the Cambridge interests, outstanding core facilities and an inclusive, supportive working The CIMR Proteomics Facility...