Do you want to contribute to top quality medical research?
At the Department of Oncology-Pathology basic, translational and clinical
research and educational activities related to cancer is carried out.
Approximately 300 people from over 40 nations are currently working at the
department. About 30 research groups with various cancer research profiles are
involved and we have around 110 PhD students. The Department of Oncology-
Pathology is responsible for undergraduate courses in Pathology, Oncology and
Forensic Medicine for medical students, as well as for Tumor biology courses
for biomedicine students and Pathology courses for opticians.
The position is within the regional and the national core facilities led by
Professor Janne Lehtiö, located at the Science for Life Laboratory in Solna.
The regional core facility is tasked with aiding users of Swedish universities
and industry with mass spectrometry (MS) based proteomics projects. The
national facility (SciLifeLab/BioMS) offers infrastructure for biological
mass spectrometry (www. bioms.se). Combined, the regional and national
facilities offer a unique research infrastructure and expertise with focus on
MS-based proteomics and proteogenomics. The core facility works in close
collaboration with Prof. Janne Lehtiö's research group, where MS-based
proteomics methods are constantly developed and applied to studies aiming at
improving individualized cancer therapy. This collaboration is a prerequisite
for the core facility's ability to offer services at the forefront of MS based
We are now looking for a PhD-level research engineer with experience of core
facility work or equivalent to join the team working in the Mass
Spectrometry core facility.
Learn more about the Lehtiö lab and Clinical Proteomics Mass Spectrometry Core
The responsibilities accompanying this position include sample preparation,
bio analytical work, analysis and reporting of protein/peptide expression,
method development, use/optimization of the MS instruments, data analysis and
data interpretation. Experiences in method development, data analysis and
reporting are important.
The role also includes reporting analysis results to facility users, taking
part of undergraduate/postgraduate teaching/training and representing the
facilities at national and international meetings and conferences.
The research engineer will be part of a research infrastructure, working
closely with project scientists, postdoctoral fellows, PhD-students,
undergraduate students, and external users/customers within academia and
industry. Important aspects of the job include:
Preparation of clinical and in-vitro samples for proteomics
Experimental analysis of samples by mass spectrometry
Compiling and analyzing proteomics data
Continuous contact with customers by email and phone
The work involves a combination of flexible and routine tasks and places a
great emphasis on independence, initiative, precision, and planning. The
position involves a variety of tasks in a stimulating research environment.
Nice and helpful colleagues contribute to a good working environment within
A large part of the job may be contact with customers/researchers from a wide
range of fields, which is an exciting, interesting and instructive part of the
work. The facility/research group is located at the Science for Life
Laboratory with spacious and modern facilities.
We are seeking a highly motivated research engineer to be part of our mass
spectrometry core facility.
The successful candidate needs to meet our requirements.
PhD degree in analytical chemistry, biochemistry, biotechnology or in a
Years of experience in core facility work
Extensive hands-on experience of laboratory work and ability to follow
Experience with techniques applied to sample preparation for proteomics,
including protein extraction, clean-up ( e.g. by solid phase
extraction), protein concentration measurement, as well as protein
depletion and/or equalization methodologies and protein and peptide
separation/fractionation using gel-based electrophoresis (1D, 2D and
Hands-on experience with Single-Pot Solid-Phase-enhanced Sample
Preparation (SP3) and in gel/in solution protein reduction/alkylation
and protein digestion techniques
Practical experience in liquid chromatography mass spectrometry (LC-MS)
Experience in Mass spectrometry Maintenance and Troubleshooting
Experience in quantitative MS-based proteomics (TMT10 plex, TMT16 plex)
Good knowledge in methods used for analysis of omics data
Excellent communication and organizational skills
Good knowledge (both speaking and writing) of English
Competence/experience in MS-based clinical proteomics research
Competence/experience in cell culture.
Competence in sample preparation and mass spectrometry analysis of native
peptides and immunopeptidome
Collaborative experience with hospitals and clinical sample handling.
Data management and data services
Strong interests of working in centralized shared research infrastructures
We place great emphasis on personal suitability. To succeed in the role, it is
important that the applicant has a personal drive and flexible mind to handle
the rapidly developing field of proteomics and the need of custom-based
solutions in many projects. The applicant should be responsible, appreciate
daily detailed work as well as have the ability to see the big picture. The
applicant should be an effective communicator, have good organizational
skills, and be able to prioritize at times when the workload is high. The
applicant should be able to collaborate with people of different backgrounds
and at different levels, both within the group and with users from various
What do we offer?
Karolinska Institutet is one of the world's leading medical universities. Our
vision is to pursue the development of knowledge about life and to promote
better health for all. At Karolinska Institutet, we conduct innovative medical
research and provide the largest range of biomedical education in Sweden.
Karolinska Institutet is a state university, which entitles employees to
several benefits such as extended holiday and a generous occupational pension.
Employees also have free access to our modern gym and receive reimbursements
for medical care.
Welcome to apply at the latest 30th of November.
The application is to be submitted through the Varbi recruitment system.
Permanent positions is initiated by a six months trial period.
Want to make a difference? Join us and contribute to better health for
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||30.Nov.2021 11:59 PM CET