Research Asst III

Temple University

United States

November 10, 2021


Research Asst III - (21003274)


The Research Assistant III will be working for the Lewis Katz School of Medicine Center for Translational Medicine with the Gene Therapy/Viral Vector Core team. Under general supervision, performs complex research activities following established methods and procedures in support of the research project. The Research Assistant III assists with various experiments; testing standard research methodologies, procedures, and verbal and written instructions that will include vector cloning, propagation, and purification of high titer virus preparations.

Responsibilities include: Sterile technique as it relates to cell and tissue culture as well as bacterial culture growth; handling of large numbers of cell culture plates/flasks per week; use of selection to generate cell lines in cell culture; freezing and thawing cell lines and bacteria; testing cell lines for bacteria including mycoplasma; large and small-scale isolation of plasmid DNA from bacteria; using antibodies for fluorescent immune-histochemistry and Western blotting; analysis of plasmids by restriction enzyme digestion.

The Research Assistant III also maintains and cleans sophisticated laboratory instruments and equipment, inventories chemicals, supplies, and equipment, and instructs and assists students and lower-level technicians. Performs other duties as assigned. Required Education and Experience: Bachelor's degree in science-related area of research and at least one year of directly related laboratory experience. An equivalent combination of education and experience may be considered.

Required Skills and Abilities: •Ability to understand the growth rates of multiple cell lines. •Proficiency in methods of DNA transfection of cells in culture. •Proficiency in MS Office Suite, Google Calendar, and Google Drive •Demonstrated proficiency in the above-listed laboratory techniques •Excellent verbal and written communication skills •Possess the ability to build and maintain professional relationships

This is a grant funded position

This position is temporarily assigned a hybrid work arrangement (on-campus and remote), the duration of this hybrid work arrangement is at the discretion of Temple University and the Department.

Temple University values diversity and is committed to equal opportunity for all persons regardless of age, color, disability, ethnicity, marital status, national origin, race, religion, sex, sexual orientation, veteran status or any other status protected by law.

In accordance with the City of Philadelphia's mandate, all Temple University students, faculty, staff and contractors must be fully vaccinated for COVID-19 by October 15, 2021, unless they have a medical or religious exemption approved by the university. An individual is considered fully vaccinated 2 weeks or more following the second dose of the Pfizer or Moderna vaccines, or 2 weeks or more following a single dose of the Johnson & Johnson vaccine. Individuals who do not meet the University's vaccination or exception requirement by October 15, 2021 will be prohibited from working until proof of vaccination or an exception is provided. For more information visit the university's Vaccines web page at https: // www.

Compliance Statement: In the performance of their functions as detailed in the position description employees have an obligation to avoid ethical, legal, financial and other conflicts of interest to ensure that their actions and outside activities do not conflict with their primary employment responsibilities at the institution. Employees are also expected to understand and be in compliance with applicable laws, University and employment policies and regulations, including NCAA regulations for areas and departments which their essential functions cause them to interact.

To obtain additional information about Temple University please visit our website at www.

Temple University's Annual Security and Fire Safety Report contains statistics, policies, and procedures related to campus safety and can be found at: https: //

You may request a copy of the report by calling Temple University's Campus Safety Services at 215-204-7900.

Primary Location

: Pennsylvania-Philadelphia-Health Sciences Campus (HSC)-Medical Education and Research Building


: Staff


: Full-time


: Day Job

Employee Status

: Regular

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