Posting Number PG190622PD
Position Number 00109045
Position Type Post Doc
Department ID 194001 - Dept Molecular Biomedical Scie
Department 194001 - Dept Molecular Biomedical Scie
Job City & State Raleigh, NC
Essential Job Duties
This post doctoral scholar position will primarily be engaged in research into
mast cell biology and signaling pathways involved in mast cell-mediated
diseases including mastocytosis and allergic diseases. In addition, the post
doc will carry out research into lung structural cell and immune cell
interactions and signaling pathways involved in airway physiology. The post
doc must be able to work independently, but must also be able to integrate
into a multidisciplinary research environment that includes multiple research
groups and collaborations in bioengineering and chemistry.
The college is committed to practicing inclusivity, making community a
priority and promoting innovation. We embrace effective leadership and we
champion personal and professional growth.
Duties and Responsibilities
The Cruse lab is seeking a postdoctoral scholar to join the team and carry
out research projects on examining mast cell signaling and molecular biology.
The goal of our research program is to establish novel regulators of mast cell
signaling and function, and to develop novel targeted therapeutics for mast
cell-mediated diseases. The laboratory has expertise in in vitro, ex vivo and
in vivo approaches of studying molecular immunology, cell signaling and mast
cell biology in inflammation and mastocytosis. We utilize molecular and
translational medicine approaches to identify potential innovative
therapeutics that target mast cell function and allergic inflammation, as well
as mast cell neoplasms. Overall, we strive to better understand the molecular
mechanisms underlying mast cell-mediated diseases. We have a particular
interest in identifying associations between proteins and genes with asthma
pathogenesis and identifying the functions of novel gene candidates in human
lung and cord blood-derived cells. We are achieving these goals through
interdisciplinary collaborations with both clinical and basic scientists in
various related fields.
The incumbent independently performs research on human and murine mast cells
and other immune cells through the utilization of in vitro molecular and cell
biology methods. The incumbent will also perform cell isolations and cultures
from tissue, including lung tissue, and perform in vivo models of allergic
inflammation and mastocytosis (mice models).
Oversee and maintain the lab tissue culture and maintain ongoing cell cultures
of both primary cells and cell lines.
Isolate human cells from primary tissues and blood products.
Independently design, perform and analyze cellular and molecular biology
Independently design, perform and analyze research on human, murine and canine
mast cells through the utilization of cell cultures and in vivo models (mice
Isolate human mast cells from lung tissue.
Isolate and culture mast cells from blood.
Develop research methods and generate research results that will contribute
toward publications and presentations.
Teach students to advance their understanding of the subject matter and add
to/increase current laboratory procedures skills.
Train visiting researchers, students and staff in biosafety and laboratory
Performing work associated with research grants and patents, gathering
preliminary data for grants and patents, writing and preparing manuscripts for
publication, presenting research at conferences, assisting in graduate student
training, ordering supplies and other laboratory operations.
Other duties as assigned by faculty mentor.
Requirements and Preferences
Work Schedule Monday - Friday, 8am - 5pm; some weekends and holidays may be
Department Required Skills
Ph.D. or equivalent doctorate (e.g., M.D., D.V.M., Sc.D.) in appropriate
Preferred Years Experience, Skills, Training, Education
Extensive experience in cell biology, tissue/cell culture and aseptic
technique are essential. Experience with isolation and culture of cells from
tissues is essential. Experience in RT-PCR, cell transfection, cloning
techniques, molecular biology and Western blots are preferred. Experience with
mouse models preferred.
This position requires excellent writing and communications skills and the
researcher is expected to publish their findings in acceptable peer-reviewed
publications and present their work at national and international meetings.
Laboratory experience in human lung mast cell isolation and culture, primary
human airway smooth muscle, fibroblast and airway epithelial cell culture
(including differentiated ALI culture models), RNA isolation and
quantification, quantitative PCR, protein extraction and quantification,
ELISAs, Western blotting and immunohistochemistry on lung sections would be
preferred. Experience in calcium signaling and electrophysiology, as well as
molecular biology, transfection methods and cloning preferred
Required License or Certification
Valid NC Driver's License required? No
Commercial Driver's License Required? No
Anticipated Hiring Range $50,000 - $55,000 commensurate with experience
If you anticipate multiple hires from this search, please indicate how many
Job Open Date 08/02/2022
Quick Link https: // jobs.ncsu.edu/postings/155553
NC State University is an equal opportunity and affirmative action employer.
All qualified applicants will receive consideration for employment without
regard to race, color, national origin, religion, sex, gender identity, age,
sexual orientation, genetic information, status as an individual with a
disability, or status as a protected veteran.
If you have general questions about the application process, you may contact
Human Resources at (919) 515-2135 or firstname.lastname@example.org. Individuals
with disabilities requiring disability-related accommodations in the
application and interview process, please call 919-515-3148.
Final candidates are subject to criminal & sex offender background checks.
Some vacancies also require credit or motor vehicle checks. Degree(s) must
be obtained prior to start date in order to meet qualifications and receive
NC State University participates in E-Verify. Federal law requires all
employers to verify the identity and employment eligibility of all persons
hired to work in the United States.
Required fields are indicated with an asterisk ().
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