Postdoc Position – Molecular mechanism of SARS-CoV2 replication – starting October 2022

Institut Pasteur

France

October 8, 2022

Description

PROJECT: Coronaviruses manipulate many cellular pathways to promote their replication. One of the most intriguing morphological changes in infected cells is the appearance of double-membrane vesicles (DMVs), which serve as factories to produce viral genomic RNA and viral mRNAs. Shortly after infection, hundreds of such vesicles are produced. They originate from the endoplasmic reticulum by yet unknow molecular mechanisms. The image shows DMVs in an infected Vero E6 cell.

We identified host factors that are used by the virus to promote the production of these replication compartments. We generated knock out cell lines of these factors and found that replication of SARS-CoV2 is entirely arrested. The image shows very few structures that resemble DMVs, however, no viral RNA is produced in these compartments. The goal of the project is to characterize the interaction of host and viral proteins, to identify binding sites and to develop peptide-based inhibitors of the interaction to inhibit viral replication.

FUNDING AND SCIENTIFIC ENVIRONMENT: The project is funded by the Institut Carnot Pasteur Microbes et Santé. The position is available from October 2022 and the initial appointment will end in Dec. 2023. We have already considerable preliminary data, which allow us to complete the molecular characterization of the interaction within this period. A follow up study focusing on translational aspects and drug development is envisioned. The recruited postdoc will profit from a world-class research environment at the Institut Pasteur, the vibrate and stimulating Pasteur Campus, top class infrastructure including access to world class facilities (a collaboration with the EM facility and the cryo-electron tomography facility is part of the project). To learn more about our research, please visit our laboratory web page:

Membrane Biochemistry and Transport

or our Twitter account: Tweets by WollertLab

SKILLS: We are seeking motivated researchers with skills in infection biology and/or cell biology, preferentially with experience to work in biological safety level 2 or 3 facilities.

Please send your CV with motivation letter and contact details of three referees to: thomas.wollert@pasteur.fr.

Similar Jobs

Add to favorites Read more...

Postdoctoral Position In Cell Biology, Membrane Dynamics And Epithelial Homeostasis

Research Field: Biological sciences › Biology work on the role of membrane contact sites and autophagy interplay in the focused on molecular and cellular aspects of stress response and autophagy Key-words: autophagic machinery, contact sites,...

Institut Pasteur

France Aug 27, 2022

Add to favorites Read more...

Postdoctoral position in Chromatin and Infection

laboratory at the Institut Pasteur in Paris. In the laboratory we study how epigenetics contribute to establishing the invasive infections begin, how asymptomatic colonizations are maintained, and We have integrated histone modifications in phenotypic responses...

Add to favorites Read more...

Phd Student In Molecular Genetics

Research Field: Biological sciences › Biology IBDM is a joint research unit under the team " Host pathogen interaction in the Drosophila model" directed by Our body hosts billions of bacteria that colonize our skin...

Add to favorites Read more...

Post-Doc

Research Field: Biological sciences › Biology Location: Greece › Heraklion The mitotic spindle is a microtubule-based apparatus that is responsible Defects in spindle formation can novel phosphorylation sites on tubulin, which are essential for optimal...

Add to favorites Read more...

Phd Student In Biology (M/F)

Research Field: Biological sciences Location: France › MONTPELLIER associated with a decreased number of PC on colonic fibroblasts (Rocha et al. of PC and associated pathways in CRC. in the regulation of intestinal homeostasis and...

University of York

United Kingdom Sep 2, 2022

Add to favorites Read more...

Research Associate in Haematology/Cancer Biology

Research Associate in Haematology/Cancer Biology Role Description Department Associates to join the group of Professor Ian Hitchcock based in the Department of Biology at the University of York. understanding the mechanisms of cytokine signalling in...

Add to favorites Read more...

Structure Of The Vaccinia Virus Dna Replication Machinery In Complex With Dna Substrates By Single-...

poxvirus DNA replication machinery, now mainly by single-particle cryo- assemblies by cryo-EM with bound DNA substrates, nucleotides, inhibitors and experiences in the structure determination and biophysical characterization of the partners and their domains, our recent...

Institut Pasteur

France 3 days ago

Add to favorites Read more...

Postdoctoral Position offer Microbiology at Institut Pasteur, Paris

of Anaerobic Bacteria at Institut Pasteur, Department of Microbiology. signals including differences in O2 tension and oxidative stress to good model to decipher the impact of environmental pressures on the gut colonization and infection processes...

Add to favorites Read more...

M/W Post-Doctoral Fellow In Immuno-Virology

Research Field: Physics - In vitro and in vivo analyses of an immune response in cellular and animal tumor models treated by different schemes of dual therapy including proton of in vitro and in vivo...