Background: Notch proteins are master regulators of cell fate during
development and control cell renewal and differentiation in most adult
tissues. Activation of Notch proteins is regulated by successive enzymatic
cleavages that release the cell membrane-bound form that acts as a
transcriptional regulator. More recently, import roles of intracellular
vesicles in regulating Notch activity are being identified (see Pubmed
34572582). We have now identified metal-binding transporters (DMT1) as key
mediators of intracellular Notch trafficking and activity.
Key objectives. To identify the importance of metal transport on
intracellular Notch localization and activity in vesicles and how this can
block Notch activity in tumours or promote regeneration of normal cell types.
You will apply gain and loss of function (CRISPR, siRNA pharmacological) in
Notch-dependent 2D and 3D models (cell lines, normal and tumour organoids
(lung, intestine) and high-resolution fluorescent imaging (confocal, STED)
and single-cell analysis to decipher how Dmt1/Notch signaling allocates cell
identify and how iron transport is involved. You will use reagents we
developed (Ab's, Tg mouse models) to track the fate of Notch activity in
fixed and live tissues.
We are looking for a Postdoc with a PhD in molecular and cell biology and a
broad experience and track record with primary cell culture and cellular
imaging using confocal fluorescence microscopy. You will be working in a team
with a strong expertise in Notch biology.
General Profile: We are looking for an open-minded, independent and
result-oriented scientist to join our team with a strong interested in
fundamental research into regenerative medicine. You are fluent in English,
both in writing and speech.
Conditions of employment
Fixed-term contract: 3 years.
The full-time position is offered for three years. The salary will be set in
post-doc salary scale of the Collective Labor Agreement of the Dutch
Universities (between €3.836,00 and €4.474,,00 gross per month depending on
the level of experience). On top of this, there is an 8% holiday and an 8.3%
The terms of employment of Maastricht University are set out in the Collective
Labor Agreement of Dutch Universities (CAO), supplemented with local UM
provisions. For more information look at the website
www. maastrichtuniversity.nl > Support > UM employees.
Maastricht University is renowned for its unique, innovative, problem-based
learning system, which is characterized by a small-scale and student-oriented
approach. Research at UM is characterized by a multidisciplinary and thematic
approach, and is concentrated in research institutes and schools. Maastricht
University has around 20,000 students and 4,700 employees. Reflecting the
university's strong international profile, a fair amount of both students and
staff are from abroad. The university hosts 6 faculties: Faculty of Health,
Medicine and Life Sciences, Faculty of Law, School of Business and Economics,
Faculty of Science and Engineering, Faculty of Arts and Social Sciences,
Faculty of Psychology and Neuroscience. For more information, visit
GROW Institute for Oncology and Developmental Biology
Maastricht University, GROW Institute for Oncology and Developmental Biology
(www. grow-um.nl) . The radiotherapy department (www. maastrolab.com)
conducts fundamental and translational research and is closely affiliated with
Maastro patient clinic and the Maastricht Comprehensive Cancer Centre. Our
research is focused on identifying therapeutic vulnerabilities in the tumour
microenvironment to improve treatment response. The lab has four PI's with
research in the area of tumour cell metabolism (Kampen Group), Cell death
(Bock group), Extracellular vesicles (Rouschop group) and Notch and Stem
cells (Vooijs Group). In addition, we have established a strong expertise in
culturing normal, and tumour stem cell models (organoids. ALI) and have
access to a large tissue biobank and material from clinical studies. We have
excellent high resoltuion optical microscopy and regenerative medicine.
Additional information: Marc Vooijs firstname.lastname@example.org