The project aims at analyzing the formation of LLPS condensates of TopBP1 in vitro and/or in cellulo. TopBP1 (Topoisomerase IIß-binding protein) is a hub protein in human cells that functions at the crossroad of multiple vital cellular processes and assembles nuclear condensates to switch on ATR signaling (see ref.4).
Our group at the Center for Structural Biology (CBS) at the University of Montpellier (South of France) offers one open Postdoc position (18 months) to work on lipid-lipid phase separation (LLPS), a new paradigm in cell compartmentalization, using atomic force microscopy (AFM) including high- speed modality (see ref.1) and correlative AFM-fluorescence microscopy (see ref.2-3).
We are an international team at an interdisciplinary research center with excellent in-house access to state-of-the-art biophysics infrastructure and biochemistry and cell culture facilities. Moreover, several groups at the CBS are working on LLPS, which fosters creativity and collaborations. The research of PE Milhiet's group focuses on the development and use of advanced microscopies (AFM and single molecule fluorescence microscopy) to decipher the molecular mechanisms associated to the organization and remodeling of biological membranes. Our team is pluridisciplinary and counts 15 people including 8 permanent researchers. CBS is an Institute dedicated to research at the forefront of structural biology, biophysics and Bioengineering. It also proposes facilities in these fields. Montpellier is a stunning city on the French Riviera with a significant international research community.Eligibility criteria
The project is highly interdisciplinary, at the interface of biophysics, cell biology and biochemistry. Therefore, successful applicants are expected to have training and research experience in physics/biophysics, especially advanced skills in AFM. Previous experience in fluorescence imaging (confocal/TIRF/Super Resolution/FLIM), Optics as well as biochemistry, cell biology and computation (e.g. Python) skills will be positively considered. The candidates should have a solid publication record (at least one first author paper). Applicants are expected to have defended their PhD thesis within the last 2 years (< 2 years postdoctoral experience).Additional comments
References 1) Nasrallah H. et al. J Mol Biol 1886:45, 2019. 2) A. Vial et al. Nanoscale, 13(29):12484, 2021. 3) T. Fernandes et al. Scientific Reports, 10(1):7098, 2020. 4) Frattini C et al. Mol Cell. 81(6):1231, 2021.
For more information: "Integrative Biophysics of Membrane" group https: // integrativebiophysicsofmembranes.wordpress.com/ https: // twitter.com/MembranesBiophyWeb site for additional job details
https: // emploi.cnrs.fr/Offres/CDD/UMR5048-FLOLEP-044/Default.aspxRequired Research Experiences
Chemistry › Biochemistry
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Physics › Biophysics
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Biological sciences › Biological engineering
1 - 4Offer Requirements
Chemistry: PhD or equivalent
Physics: PhD or equivalent
Biological sciences: PhD or equivalent
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