Description

Organisation/Company: CNRS

Research Field: Biological sciences Environmental science

Researcher Profile: First Stage Researcher (R1)

Application Deadline: 13/07/2022 23:59 - Europe/Brussels

Location: France › GRENOBLE

Type Of Contract: Temporary

Job Status: Full-time

Hours Per Week: 35

Offer Starting Date: 01/10/2022

The candidate will be part of the Photosymbiosis team (photosymbiosis.com) of the LPCV -laboratory of Cellular and Plant Physiology- (UMR 5168 - CNRS -CEA - Univ. Grenoble Alpes - INRA) within the IRIG institute of the CEA in Grenoble. The projects of the Photosymbiosis team aim to better understand the functioning of interactions between unicellular organisms (photosymbiosis, parasitism) by combining subcellular microscopy approaches (nanoSIMS, Synchrotron X-Ray fluorescence, 3D electron microscopy). Locally, the project will be in close collaboration with the IRIG institute: INAC platform (IRIG) and the Institute of Structural Biology (IBS, Grenoble).

Missions : Host-parasite interactions are widespread in single-celled marine plankton. The main objective of the project will be to better understand the trophic strategies of the parasite during its intracellular development in the host (dinoflagellate) using subcellular imaging techniques (nanoSIMS coupled to isotope labeling and 3D electron microscopy) and metabolomics (lipidomes). Moreover, to better understand the metabolism and infection mechanisms of the free form of the parasite, the second objective of the project will be to reveal the 3D ultrastructure of the dinospores released after infection with 3D electron microscopy approaches (FIB-SEM and cryo- tomography). Activities : - Culture of the microalgae (host) - parasite system in the laboratory - Photo-physiological analyses (photosynthesis, carbon fixation) - Incubation with stable isotopes coupled to nanoSIMS - 3D electron microscopy (FIB-SEM and cryo-electron tomography) - Image analysis (segmentation, 3D reconstruction) - Communication through international meetings and workshops.

Background and skills : - Training in biology - Knowledge and interest in cellular interactions: mutualistic symbioses or parasitism - Expertise in microalgae culture and physiology will be appreciated - Knowledge and expertise in sample preparation for 3D electron microscopy (FIB-SEM, cryo-TEM), and image analysis for 3D reconstruction. - Ability to work independently in an interdisciplinary and international environment (fluent in English), and to interact in a young and dynamic research group.

Web site for additional job details

https: // emploi.cnrs.fr/Offres/Doctorant/UMR5168-JOHDEC-008/Default.aspx

Required Research Experiences

RESEARCH FIELD

Biological sciences

YEARS OF RESEARCH EXPERIENCE

None

RESEARCH FIELD

Environmental science

YEARS OF RESEARCH EXPERIENCE

None

Offer Requirements

REQUIRED EDUCATION LEVEL

Biological sciences: Master Degree or equivalent

Environmental science: Master Degree or equivalent

REQUIRED LANGUAGES

FRENCH: Basic

Contact Information

Organisation/Company: CNRS

Department: Laboratoire de physiologie cellulaire et végétale

Organisation Type: Public Research Institution

Website: https:// irtsv.cea.fr/dsv/irtsv/Pages/PCV/Accueil.aspx

Country: France

City: GRENOBLE