The Institute of Molecular Genetics of Montpellier (IGMM) is a joint research unit of CNRS and the University of Montpellier, with 200 people divided into 17 research groups, 9 common services (5 of which are shared with other units of the CNRS campus) and 9 technological and scientific platforms. The IGMM is a multidisciplinary institute whose work has an international impact in molecular and cellular biology (www. igmm.cnrs.fr). The PhD student will be assigned to the new team, which was created within the IGMM a year ago and whose theme is "RNA viruses and host factors", directed by Karim MAJZOUB.
The thesis subject focuses on the study of restriction factors opposing the replication of Hepatitis Delta virus (HDV) and the Deltavirus family in general. A genetic screen focused on ISGs (Interferon Stimulated Genes) family will be performed to identify genes that would negatively regulate viral genome expression. The contribution of the identified candidates in Deltavirus replication will then be studied by gene depletion and overexpression methods (cDNA, CRISPR), microscopy (smFISH, Immunofluorescence), Western blot, qPCR and Northern blot. These techniques will be performed in several animal cell lines (human, rodent, snake) as well as in a mouse animal model. The aim of the project is to identify and characterize ISGs suspected of inhibiting Deltavirus replication. This work will provide important fundamental knowledge regarding animal immunity to the Deltavirus family. Moreover, this knowledge could lead to new applied antiviral strategies to fight against this family of viruses, against which there are no effective antivirals to date.Web site for additional job details
https: // emploi.cnrs.fr/Offres/Doctorant/UMR5535-SARADE-025/Default.aspxRequired Research Experiences
Biological sciences: Master Degree or equivalent
Medical sciences: Master Degree or equivalent
Pharmacological sciences: Master Degree or equivalent
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