1 Nov 2023
Job InformationOrganisation/Company
CNRS
Department
Bioénergétique et ingénierie des protéines
Research Field
Chemistry » Biochemistry
Physics » Biophysics
Biological sciences » Biological engineering
Researcher Profile
First Stage Researcher (R1)
Country
France
Application Deadline
21 Nov 2023 - 23:59 (UTC)
Type of Contract
Temporary
Job Status
Full-time
Hours Per Week
35
Offer Starting Date
1 Jan 2024
Is the job funded through the EU Research Framework Programme?
Not funded by an EU programme
Is the Job related to staff position within a Research Infrastructure?
No
Offer DescriptionIn the BIP laboratory, the different teams are interested in the different aspects of the adaptation of microorganisms to their environment by enzymology approaches specific such as biochemistry, biophysics (NMR, EPR, electrochemistry). In particular, the assimilation and acquisition of CO2 coupled to bioenergy process are deciphered on several organisms prokaryotes and eukaryotes.
The general objective of the PhD project is to decipher the regulation of enzymes of the acquisition and assimilation of CO2 in the diatom Phaeodactylum tricornutum. Photosynthesis occurs in the chloroplast of diatoms, and their molecular properties differ from that of other models (chloroplast of viridiplantae or cyanobacteria). CO2 is accumulated in the chloroplast by a CO2 concentration mechanism and its actors are bicarbonate transporters and carbonic anhydrases. Among them, the diatoms possess a peculiar carbonic anhydrase : the iota-CA, that is active without metallic cofactor, and that is expressed upon change in the culture conditions from high to atmospheric or low CO2. The objective of the PhD candidate is to determine the enzymatic properties of iota-CA, and its cofactor. The PhD candidate will set up new CA activity assays and will use wild type and mutant recombinant enzymes (already produced in the lab). In parallel, the partners of this CA will be identified by biochemistry approaches (pool down). CO2 is assimilated by the Ribulose 1,5 Bisphophate Carboxylase/Oxygenase (RuBisCO), and its substrate is renewed by the Calvin Benson Bassham cycle (CBB). The regulation of some CBB enzymes such as phosphoribulokinase or glyceraldehyde 3-phosphate dehydrogenase differ in diatom compared to in viridiplantae. The PhD candidate will use established activity assays to determine the molecular properties of these enzymes and the conditions of their activation (pH, redox, cofactors). The PhD candidate will compare the catalytic activity of recombinant enzymes compared to that in cell extracts. Hypothetical supramolecular complexes will be searched by pool down approaches and their interacting partners will be identified. In a second period, the PhD candidate will use wild type and mutant diatoms (deleted of regulatory proteins or of iota-CA) obtained by the project AlgAdvance in order to compare the amount of metabolite of photosynthesis by NMR in collaboration by the BIP02 group. A comparative proteomic approach will be also employed to compare the metabolic root of the wild type and mutants cells, in collaboration by the proteomic plateform of the IMM.
RequirementsResearch Field
Chemistry
Education Level
Master Degree or equivalent
Research Field
Physics
Education Level
Master Degree or equivalent
Research Field
Biological sciences
Education Level
Master Degree or equivalent
Languages
FRENCH
Level
Basic
Research Field
Chemistry » Biochemistry
Years of Research Experience
None
Research Field
Physics » Biophysics
Years of Research Experience
None
Research Field
Biological sciences » Biological engineering
Years of Research Experience
None
Website for additional job details
https: // emploi.cnrs.fr/Offres/Doctorant/UMR7281-AURBIM-025/Default.aspx
Work Location(s)Number of offers available
1
Company/Institute
Bioénergétique et ingénierie des protéines
Country
France
Website
https: // emploi.cnrs.fr/Candidat/Offre/UMR7281-AURBIM-025/Candidater.aspx
ContactWebsite
https:// bip.cnrs-mrs.fr
STATUS: EXPIRED