Description

A vascular biology and cardiovascular genetics laboratory in Vascular Surgery within the Department of Surgery seeks a full-time Laboratory Manager (Life Science Research Professional 1) to conduct research in the field of atherosclerosis and aneurysm research.

The LSRP will perform at a very high level tasked with conducting advanced vascular biology studies employing molecular biology and cell culture models. The LSRP will be involved in specific research projects that are funded by the NIH and other funding agencies, and will independently handle experimental design, execution, and interpretation of results. They will also be expected to make original contributions to the ongoing scientific efforts, analyzing their data and drawing inferential conclusions that promote further research. This LSRP will also be expected to track animals involved in various complex animal model studies, perform genotyping and molecular and in situ studies investigating gene expression and disease features. This LSRP will be responsible for the generation of mouse genetic models and will maintain the mouse colony breeding.

They will be expected to develop figures and text for manuscripts and grants, which they will help generate and submit, as well as supply technical text related to the methods, findings, and implications of the research. The LSRP will be expected to independently develop protocols for new methods in the lab that are central to the research mission, to document and to teach these protocols to incoming students and fellows. The position also entails top level lab management which will support such tasks related to ongoing operation of the lab, including ordering supplies and equipment, annual grant reports, maintaining safety records and lab equipment, and adhering to safety regulations and compliance and regulatory protocols.

Additional duties and responsibilities will include, but are not limited to:

Perform experiments and optimize experimental protocols; train and assist lab personnel and students with experiments as needed. Compile and analyze data, interpret findings, and help prepare data for posters and presentations. Work will include diverse molecular biology assays, cell culture experiments, quantitative microscopy, histopathology section processing and analysis, and other related work in the field of vascular biology. Experience with RNAseq/bioinformatics is desired.

Establish Standard Operating Procedures (SOPs) for all lab equipment and existing and new technologies in the lab, troubleshoot, schedule preventative and routine maintenance and calibration of equipment, microscopes, and pipettes. Create reminder calendar for routine maintenance.

Cell culture, including routine mycoplasma testing for all cell lines used in the lab. Implement protocols for routine incubator and biosafety hood cleaning (duties to be shared with other lab members).

Animal husbandry work, including colony maintenance, weaning, genotyping and long term breeding of complex cell-specific knockout models.

Supervise and train new staff, postdocs or students. This includes oversight and instruction on techniques as well as consultation on project work. Serves as technical resource for other research staff.

Assist PI with grant writing, annual reports, material transfer agreements, and other lab-related paperwork. Manage Biosafety regulatory protocols. Package and ship/arrange receipt of samples to/from collaborating laboratories. Perform all ordering for the team. Ensure laboratory is in compliance with all Stanford policies and that all laboratory personnel have completed required training.

- Other duties may also be assigned.

DESIRED QUALIFICATIONS:

Bachelor's degree in cellular or molecular biology, immunology or related scientific field.

Advanced degree preferred.

Related laboratory research experience.

Knowledge of molecular and cell biology principles and assays.

Experience with nucleic acid isolation and cell culture.

Experience with molecular biology lab equipment (i.e. Nanodrop, Agilent Bioanalyzer, PCR/qPCR, fluorescence microscopy, FACS).

Ability to create and maintain inventory databases for reagents, consumables, and banked specimens.

Must be organized, keep excellent records.

Have excellent communication and interpersonal skills.

Microsoft Word, Powerpoint and Excel proficiency.

Previous supervisory experience.

Willingness to learn animal surgery models is desired, but not mandatory.

EDUCATION & EXPERIENCE (REQUIRED):

Bachelor's degree in related scientific field.

KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):

General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.

Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.

Ability to maintain detailed records of experiments and outcomes.

General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.

Ability to work under deadlines with general guidance.

Excellent organizational skills and demonstrated ability to accurately complete detailed work.

PHYSICAL REQUIREMENTS:

Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.

Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.

Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.

Must have correctible vision to perform duties of the job.

Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.

Ability to lift heavy objects weighing up to 50 pounds.

Ability to work in a dusty, dirty, and odorous environment.

Position may require repetitive motion.

- Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.

WORKING CONDITIONS:

May require working in close proximity to blood borne pathogens.

May require work in an environment where animals are used for teaching and research.

Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.

Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.

May require extended or unusual work hours based on research requirements and business needs.

WORK STANDARDS:

Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.

Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors bases on training and lessons learned.

Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University's Administrative Guide, https:// adminguide.stanford.edu.

As an organization that receives federal funding, Stanford University has a COVID-19 vaccination requirement that will apply to all university employees, including those working remotely in the United States and applicable subcontractors. To learn more about COVID policies and guidelines for Stanford University Staff, please visit:https: // cardinalatwork.stanford.edu/working- stanford/covid-19/interim-policies/covid-19-surveillance-testing-policy

The job duties listed are typical examples of work performed by positions in this job classification and are not designed to contain or be interpreted as a comprehensive inventory of all duties, tasks, and responsibilities. Specific duties and responsibilities may vary depending on department or program needs without changing the general nature and scope of the job or level of responsibility. Employees may also perform other duties as assigned.

Consistent with its obligations under the law, the University will provide reasonable accommodation to any employee with a disability who requires accommodation to perform the essential functions of his or her job.

Stanford is an equal employment opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law.