LAB RESEARCH ANALYST I

Duke University

United States

August 21, 2021

Description

LAB RESEARCH ANALYST I

Requisition Number:: 136868

Date: Jul 16, 2021

Location:

Durham, NC, US, 27710

Personnel Area: MEDICAL CENTER

This Position is Fully Grant Funded

School of Medicine:

Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked tenth among its peers, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where traditional barriers are low, interdisciplinary collaboration is embraced, and great ideas accelerate translation of fundamental scientific discoveries to improve humanhealth locally and around the globe.

Comprised of 2,400 faculty physicians and researchers, the Duke University School of Medicine along with the Duke University School of Nursing and Duke University Health System create Duke Health. Duke Health is a world-class health care network. Founded in 1998 to provide efficient, responsive care, the health system offers a full network of health services and encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Private Diagnostic Clinic, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

DEPARTMENT OF BIOSTATISTICS AND BIOINFORMATICS

LAB RESEARCH ANALYST I

Job Code: 1888, Level 11

Lead management of a mouse colony including: complex breeding, protocols to establish and maintain colonies with three or more homozygous and/or heterozygous alleles; genotyping to identify desired mice breeding and for experiments; complex multi-organ dissections to isolate primary cell; and purification, culture, differentiation, and characterization of those primary cells. (20%)

Independently lead STARR-seq and CRISPR screening assays in those primary cells including: (i) developing, optimizing, high-throughput sequencing, and analysis of complex plasmid assay libraries; (ii) transfection and transduction of those assay libraries into primary cells; (iii) isolation of nucleic acids and generation of high-throughput sequencing libraries; (iv) sequencing of those libraries on illumina sequencing instruments; (v) evaluating and interpreting results in collaboration with the bioinformatics team; and (vi) develop and follow quarterly data production schedules for the above experiments. (50%)

Independently develop and optimize new technologies for production including: (i) new technologies for single cell CRISPR screening; (ii) new technologies for multiplexes CRISPR screens; (iii) improved technologies for high-throughput reporter assays; and(iv) assays published in other labs that could benefit lab research goals. (20%)

Train new graduate students and staff on STARR-seq assays, CRISPR screening assays, and other genomic technologies used in the lab routinely. (10%)

Education/Training

Bachelor's degree in relevant scientific field (biology, genetics, molecular biology). Two year's experience in the lab with ability to work independently.

Job Skills

Ability to work independently, as well as ability to work with a research team. Ability to manage competing priorities. Strong time management and organizational skills.

Duke is an Affirmative Action/Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex, sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essentialjob functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.

Nearest Major Market: Durham Nearest Secondary Market: Raleigh

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