The objective of this thesis is to use our technological advance in imaging
coupled with electrophysiology to revisit the LTD. In particular, we will
redefine the respective modifications in the organization and dynamics of
AMPARs in synaptic transmission during the early and sustained phases of LTD.
This will pave the way for new approaches to controlling LTD. In parallel, we
will describe the structural plasticity induced by LTD. We will then determine
the molecular link between these two plasticities.
• Use our super-resolution imaging techniques in living and fixed tissues to
characterize the organization and dynamics of AMPARs at the nanometric scale;
• Combine calcium imaging to highlight active synapses with super-resolution
imaging and electrophysiology to correlate the content of synaptic AMPARs with
the strength of the associated synaptic response;
• Use a recently characterized molecular tool allowing the immobilization of
AMPARs both in brain slices and in vivo;
• Develop a long-term fluorescence imaging technique to follow structural
plasticity both in cell culture and in brain slices.
Funding category: Contrat doctoral
PHD title: doctorat de neuroscience
PHD Country: France
Offer RequirementsSpecific Requirements
Le candidat doit avoir de solides bases en neuroscience et une forte
motivation, des compétences autour de l'imagerie et/ou de la physiologie de la
synapse sont préférables.
Uniquement des personnes ayant un master2 ou équivalent pourront être recrutés